Figure 4
From: Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer
Combined bisulfite restriction analysis in ductal carcinoma in situ and more advanced primary breast tumors. The NRN1, GFI1, HOXB13, HNF1B-associated CpG islands were analyzed by Combined bisulfite restriction analysis in ductal carcinoma in situ (DCIS) and more advanced breast carcinoma. Three representative DCIS, seven stage I tumors, five stage II tumors and five stage III breast tumors are shown. The total number of specimens analyzed per gene and the relative methylation frequencies are presented in Table 3. N/T, normal/tumor pairs; M, DNA marker; Meth. DNA, HeLa DNA methylated in vitro with SssI methyltransferase, serving as a positive control.