Figure 3
From: Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer
Methylation of an uncharacterized CpG island on chromosome 7. Six cases of ductal carcinoma in situ (DCIS) and 32 invasive breast tumors of different histological type and grade were analyzed for CpG methylation. The target CpG island (hit number 5, Table 2) was subjected to the BstUI combined bisulfite restriction analysis assay. N/T, normal/tumor pairs. Vertical arrows indicate tumor-specific methylation. Target number 201 is a stage II breast carcinoma. M, DNA marker; Meth. DNA, HeLa DNA methylated in vitro with SssI methyltransferase, serving as a positive control.