Figure 1
From: Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer
Outline of the methylated-CpG island recovery assay-assisted CpG island microarray analysis. Prior to microarray analysis, tissue sections, derived from six independent ductal carcinomas in situ (DCIS) and their matching normal areas, were stained with H&E and validated by a pathologist. Genomic DNA derived from tumor specimens and matching normal tissue was then subjected to methylated-CpG island recovery assay (MIRA) pull-down as described in Materials and methods. MIRA-enriched fractions were labeled with different dyes, mixed, and hybridized to CpG island-Agilent slides and the relative enrichment factors between different tissues were determined by statistical analysis.