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Figure 2 | Breast Cancer Research

Figure 2

From: Protein kinase D1 regulates matrix metalloproteinase expression and inhibits breast cancer cell invasion

Figure 2

PKD1 is not expressed in invasive breast cancer cell lines. (a,b) MCF-10A cells and BT-474, MCF-7, SKBR3, T47D, MDA-MB-231, 1-HMT-3522-S1 or 1-HMT-3522-T4/2 breast cancer cell lines were cultivated under normal growth conditions. mRNA was isolated and the expression of protein kinase D (PKD) 1, PKD2, PKD3 and actin was detected by RT-PCR. (c) MCF-10A cells and BT-474, MCF-7, SKBR3, T47D, MDA-MB-231, 1-HMT-3522-S1 or 1-HMT-3522-T4/2 breast cancer cell lines were cultivated under normal growth conditions. Cells were lysed and lysates were analysed for PKD1 expression by western blotting. Actin served as loading control. (d) MDA-MB-231 cells were either left untreated or treated with RG108 (250 nM, for 24 hours). PKD1 was immunoprecipitated (α-PKD1 antibody) and immunoprecipitates were analysed for PKD1 expression. Western blotting of lysates for actin (α-actin antibody) served as a control.

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