Figure 5

Macrophage chemotaxis is influenced by the denatured state of collagen 1 and fibronectin (FN) levels. (a) upper panel: Photomicrograph of transwell filter showing inhibition of J774 cell motility when 10 μg/ml fibrillar collagen 1 is added to control extracellular matrix (ECM) and used as chemoattractant. 100× magnification. (a) lower panel: Quantitation of data demonstrates significantly reduced chemotaxis towards collagen 1. *p < 0.0001. (b) upper panel: Photomicrograph showing that 10 μg/ml denatured collagen (gelatin) added to tamoxifen ECM as chemoattractant increases J774 macrophage cell motility. 100× magnification. (b) lower panel: Quantitation of data demonstrates significantly increased chemotaxis towards denatured collagen, *p < 0.0001. (c) upper panel: Photomicrograph showing that 20 μg/ml FN added to tamoxifen ECM increases J774 cell motility. 100 × magnification. (c) lower panel: quantitation of data demonstrates significantly increased chemotaxis towards FN. *p = 0.0004.