Figure 7

Erk5-RNA interference partially suppresses MCF7-MEK5 EMT gene expression. (a) MCF-7-MEK5 cells were transfected with pSUPER-scrambled (sh-VEC) or pSUPER-Erk5-RNAi (sh-Erk5) and harvested 24 hours later for Western blot analysis of Erk5 expression with actin as control. (b) MCF-7-MEK5 cells with sh-VEC or sh-Erk5 were harvested for RNA isolation and RT-PCR analysis of expression of MEK5, Erk5, E-cadherin, SNAI1, SNAI2, ZEB1, and GAPDH. MCF-7-VEC cells were analyzed for comparison purposes. (c) MCF-7-MEK5 cells expressing either sh-VEC or sh-Erk5 were harvested for RNA isolation and real-time PCR analysis for expression of SNAI2, ZEB1, CDH1, CDH2, and VIM. The data are presented as mean ± standard error of the mean from three independent experiments.