Skip to main content
Figure 2 | Breast Cancer Research

Figure 2

From: Proteomic analysis of tumor necrosis factor-α resistant human breast cancer cells reveals a MEK5/Erk5-mediated epithelial-mesenchymal transition phenotype

Figure 2

CA-MEK5-mediated resistance to TNF-α induced loss of clonogenic survival. (a) MCF-7-MEK5 and MCF-7-VEC cells were plated for clonogenic survival assay and treated with different concentrations of TNF-α (0 to 100 ng/ml) for 18 hours. Following this, cells were cultured in a fresh media without TNF-α and observed daily for 1 to 2 weeks. (b) MCF-7-MEK5 cells were plated for clonogenic survival assay and transfected with vector or DN-MEK5 (100 ng/well) for 24 hours. The following day cells were treated with vehicle or TNF-α (1 ng/ml) for 18 hours. Following this, cells were cultured in a fresh media without TNF-α and observed daily for 1 to 2 weeks. Colonies were fixed, stained with crystal violet and counted. Data are presented as percentage clonogenic survival from untreated control cells (100%) ± standard error of the mean (n = 3). MEK, mitogen-activated protein kinase kinase; TNF, tumor necrosis factor.

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us