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Table 2 Reverse library – Genes upregulation in the absence of prolactin

From: Heat shock protein-90-alpha, a prolactin-STAT5 target gene identified in breast cancer cells, is involved in apoptosis regulation

Confirmed

Observed

Function

Accession number

Protein name

  

RNA-related functions

  
  

Splicing factor

[Swiss-Prot:O75533]

Splicing factor 3B subunit 1, spliceosome-associated protein 155

  

Translational regulation

[Swiss-Prot:Q8TB72]

Pumilio homolog 2, pumilio-2, KIAA0235 fragment

  

Chaperone

  
  

Chaperone

[Swiss-Prot:O60925]

Prefoldin subunit 1

  

Protein synthesis

  

*

 

Activates the trk oncogene

[Swiss-Prot:P62424]

60S ribosomal protein L7A, surfeit locus protein 3, PLA-X polypeptide

  

Electron transport

  
  

Electron transport

[GenBank:AAC25442]

NADH dehydrogenase subunit 2 homo sapiens

  

Selenium metabolism and protection oxidative stress

[Swiss-Prot:Q99475]

Km-102-derived reductase like factor, thioredoxin reductase

  

Electron transport

[RefSeq:NP_055217] + [GenBank:EAW62308]

[GenBank:AAH90048]

[GenBank:AAH01390]

[Swiss-Prot:O14949]

Ubiquinol-cytochrome c reductase complex ubiquinone binding protein QP-C, complex III subunit VII

  

Transport

  

*

 

Vesicle trafficking protein transport

[GenBank:BAA07558]

Hypothetical protein KIAA0079, HA3543, SEC24-related protein C

*

 

Protein transport

[GenBank:CAI15005]

Coatomer alpha subunit, Alpha-COP

  

Proteasome

  

*

 

Proteasome

[Swiss-Prot:P25788]

Proteasome component C8, macropain subunit C8, proteasome subunit alpha type 3 multicatalytic endopeptidase complex subunit C8

  

Ubiquitination

  
  

Deubiquitinating enzyme tumour suppressor

[GenBank:BAA74872]

KIAA0849 protein, CYLD gene

  

Ubiquitin-specific protease cysteine proteases

[GenBank:AAH64516]

[GenBank:NP_005144]

[Swiss-Prot:Q14694]

[GenBank:ABM86690]

[GenBank:ABM83479]

KIAA0190 ubiquitin specific peptidase 10

*

 

Ubiquitin cycle

[GI:4929720]

[GenBank:NP_057490]

[Swiss-Prot:Q9Y3C8]

[GenBank:AF151884]

Ubiquitin-fold modifier conjugating enzyme 1

  

Transcription factors

  

*

 

Transcription factor

[Swiss-Prot:Q12905]

Interleukin enhancer-binding factor 2, NF45 protein

  

Transcription factor complex, RNA binding

[GenBank:CAA10029]

NS1-binding protein

  

Miscellaneous

  

*

2

N-oligosaccharyl transferase complex

[GenBank:CAB41763]

DJ343K2.2.1, ribophorin II isoform 1

  

Unknown

[Swiss-Prot:O95801]

Tetratricopeptide repeat protein 4

  

Kinase

[GenBank:BAA76815]

KIAA0971 protein, FAST kinase domains 2

  

Mitochondrial fusion

[Swiss-Prot:O95140]

[GenBank:AAD02058]

MFN2, KIAA0214 protein, CPRP1

  

Calcium binding

[Swiss-Prot:P62158]

Calmodulin

  

Glycolytic and gluconeogenesis pathways Second product-transcription factor

[Swiss-Prot:P06733]

Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, enolase 1, MBP-1, plasminogen-binding protein

 

2

Palmitoyl-(protein) hydrolase activity

[PIR:I58097]

Palmitoyl protein thioesterase precursor, EC 3.1.2.22

*

 

Iron ion transport

[GenBank:gi:37432]

Transferrin receptor, p90, CD71

*

2

Unknown OR Wnt signalling pathway

[GenBank:gi:1167502]

[GenBank:gi:1524104]

Hypothetical protein TI-227H wnt 13

  1. Prolactin-regulated genes. cDNA fragments identified preferentially in the reverse libraries (absence of prolactin) were sequenced. Sequences that were identical to those that encoded human proteins are presented using the protein accession number. Genes that were identified more than once are indicated as the number of times observed. Genes that were confirmed by a second screen are marked with an asterisk.
  2. Alpha-COP = alpha coat protein; CPRP1 = caprine prolactin-related protein-1; CYLD = cylindromatosis; FAST = Fas-activated serine/threonine; MFN2 = mitofusin-2, transmembrane GTPase; MBP-1 = C-myc promoter-binding protein; NADH = nicotinamide adenine dinucleotide plus hydrogen; NS1 = non-structural 1 protein; NF45 = nuclear factor of activated T-cells 45 kDa; wnt 13 = wingless-type MMTV (mouse mammary tumour virus) integration site family, member 2B.
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Breast Cancer Research

ISSN: 1465-542X

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