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Table 1 Forward library – Genes upregulated in the presence of prolactin

From: Heat shock protein-90-alpha, a prolactin-STAT5 target gene identified in breast cancer cells, is involved in apoptosis regulation

Confirmed

Observed

Function

Accession number

Protein name

  

RNA-related functions

  
  

mRNA splicing

[Swiss-Prot:Q8IYB3]

Serine/arginine-related nuclear matrix protein

  

mRNA transport

[Swiss-Prot:Q8N1F7]

KIAA0095 protein, Nup93

 

2

RNA-binding protein

[Swiss-Prot:P09651]

Heterogeneous nuclear ribonucleoprotein A1

  

Chaperone

  

*

5

Heat shock

[GenBank:BAA13431]

[Swiss-Prot:O75322]

Heat shock protein 90 alpha and beta

 

2

Heat shock

[GenBank:AAD11466]

[GenBank:AAD21817]

[GenBank:AAD21815]

[GenBank:AAD21816]

[Swiss-Prot:P34931]

Heat shock 70 KD protein 1 and 2 and heat shock 70 KD protein 1-like

  

Part of the TCP1 complex

[GenBank:CAG33352]

TCP-1-beta, CCT-beta

  

ER-resident chaperone

[Swiss-Prot:P06761]

GRP78, BiP

  

Protein synthesis

  

*

5

Protein synthesis

[Swiss-Prot:Q14222]

Elongation factor-1 alpha

  

Upregulated in metastasis

[Swiss-Prot:Q9NQ50]

39S ribosomal protein L40, mitochondrial precursor, L40mt, MRP-40

  

Electron transport

  

*

2

Oxidative phosphorylation

[GenBank:AAD13930]

Mitochondrial hinge protein, OXPHOS system complex III mitochondrial subunit

  

Mitochondrial electron transport chain

[Swiss-Prot:Q15070]

inner membrane protein OXA1L, mitochondrial precursor

  

Mitochondrial electron transport chain

[GenBank:BAA77673]

NADH dehydrogenase subunit 4

  

Transport

  
  

ER-to-Golgi transport

[Swiss-Prot:O75935]

Dynactin subunit 3, dynactin complex subunit 22 kDa subunit, p22

  

Proteasome

  
  

Regulatory subunit of proteasome function

[Swiss-Prot:Q13041]

26S proteasome regulatory subunit S2, p97, tumour necrosis factor type 1 receptor-associated protein 2

  

Proteasome

[Swiss-Prot:P25789]

Proteasome component C9, macropain subunit C9

  

Ubiquitination

  
  

Heterodimerising with cullin-1 to catalyse ubiquitin polymerisation

[GenBank:AAD29715]

Ring box protein 1

  

Survival

  

*

 

Survival

[GenBank:AAB87479]

TEGT protein, BAX inhibitor 1

*

2

Caspase-9 inhibition

[Swiss-Prot:O00165]

HA1-associating protein Hs1-binding protein

  

Miscellaneous

  
  

Activity in milk

[Swiss-Prot:P07195]

L-lactate dehydrogenase H chain LDH-B

 

2

ECM-receptor interaction

[GenBank:BAA76814]

KIAA0970 protein, fibronectin type III domain containing 3A

  

Toll-like receptor-mediated interferon response

[GenBank:AAD34590]

T2K protein kinase homolog (mouse)

*

2

Iron storage

[Swiss-Prot:P02794]

Ferritin heavy chain, ferritin H subunit

  

Endosome location

[Swiss-Prot:Q99805]

Transmembrane 9 superfamily protein member 2 precursor, p76

  

Regulation of nonmuscle myosin II

[Swiss-Prot:O14950]

Myosin regulatory light chain

  

nucleic acid synthesis

[Swiss-Prot:P30085]

UMP-CMP kinase 1, cytidylate kinase

  

Retaining proteins in the ER

[Swiss-Prot:Q08013]

TRAP gamma, SSR-gamma

*

 

Peptide-modifying enzyme component

[Gi:2894085]

P40 mRNA for lanthionine synthetase C-like protein 1

  1. Prolactin-regulated genes. cDNA fragments identified preferentially in the forward library (presence of prolactin) was sequenced. Sequences that were identical to those that encoded human proteins are presented using the protein accession number. Genes that were identified more than once are indicated as the number of times observed. Genes that were confirmed by a second screen are marked with an asterisk.
  2. BAX = Bcl2-associated X protein; BiP = binding protein; CCT-beta = chaperonin-containing TCP-1 complex; UMP-CMP = citidine monophosphate; ER = endoplasmic reticulum; GRP78 = 78 KD glucose-regulated protein precursor; NADH = nicotinamide adenine dinucleotide plus hydrogen; Nup93= nuclear pore complex protein; NF45 = nuclear factor of activated T-cells 45 kDa; OXA1L = oxidase assembly 1-like protein; OXPHOS = oxidative phosphorylation; TCP-1-beta = T-complex protein 1, beta subunit; TEGT = testis enhanced gene transcript; TRAP gamma = translocon-associated protein, gamma subunit; SSR-gamma = signal sequence receptor gamma subunit.
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Breast Cancer Research

ISSN: 1465-542X

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