Figure 4

Prolactin is a survival factor for HC11 cells. Differentiated HC11 cells were starved of serum and hormones and with specific hormones added back alone as indicated. After 72 hours, cytoplasmic extracts were prepared and analysed by sandwich ELISA for apoptotic mono- and oligo-nuclesomes. All rescue treatments of prolactin (P), dexamethasone (D) or insulin (I) significantly reduced apoptosis caused by starvation of hormones and serum (pos), for example, between prolactin rescue and positive control, p = 0.001 in a t-test. Negative control (neg) are cells in differentiation medium (DIP) without starvation. Each bar represents the average of three experiments with standard deviation.