Table 2 List of the oligonucleotides sequences used for quantitative real-time PCR
Gene symbol | Annotation | Primer sequences | |
|---|---|---|---|
Target genes | CGI-41 | CGI-41 protein | Forward: CCAGGCGTGCAGGGTATC Reverse: GCCCCCGCTGCACAT |
C16orf5 | Chromosome 16 open reading frame 5 | Forward: CAGCCAGAGCAGTTAGCCAGTTA Reverse: CTGACTCCAGACAACTTACCCATTC | |
GOSR2 | Golgi SNAP receptor complex member 2 | Forward:GCAGGAGAGACAGCGAGAAGA Reverse:TGCAGTGATTCGTCCATTGG | |
MARK3 | MAP/microtubule affinity-regulating kinase 3 | Forward: AGACACTCAGTGATTCAGAATGGC Reverse: GAAGCAACTGGAGTTCTCTGATCA | |
LOX | Lysyl oxidase | Forward: CAGGACATCATGCGTATGCC Reverse: CCAGGCACTGATTTATCCATTG | |
STK25 | Serine/threonine kinase 25 (STE20 homolog, yeast) | Forward: ACCTGGTGGAGCGAGTGC Reverse: TTCAGCGGGTGGATGTCAG | |
SULF-1 | Sulfatase 1 | Forward:GGCATTTTGAATCAGCTACACGTA Reverse:TCCCATCCATCCCATAACTGTC | |
TXNL2 | Thioredoxin-like 2 | Forward: GACCACAGGCGTGCACC Reverse: GATACCTTTCCTCATCCATCACAAG | |
Endogenous genes | HPRT | hypoxanthine phosphoribosyltransferase 1 | Forward: GAACGTCTTGCTCGAGATGTGA Reverse: TCCAGCAGGTCAGCAAAGAAT |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | Forward: ACCCACTCCTCCACCTTTGA Reverse: CTGTTGCTGTAGCCAAATTCGT | |
BCR | Breakpoint cluster region | Forward: CCTTCGACGTCAATAACAAGGAT Reverse: CCTGCGATGGCGTTCAC |