Figure 1

Oxamate inhibits human recombinant lactate dehydrogenase A (LDH-A) and aspartate aminotransferase (AAT) activity in vitro. (a) Recombinant enzyme assays using purified human LDH-A were performed as described. (b) Lineweaver-Burk double reciprocal plots examining LDH-A enzyme activity as a function of pyruvate concentration. Kinase assays were performed in the presence or absence of 80, 240 or 800 μM oxamate. (c) In vitro recombinant enzyme assays using purified human AAT were performed as described. (d) Lineweaver-Burk double reciprocal plots examining AAT enzyme activity as a function of α-ketoglutarate (KG) concentration. Kinase assays were performed in the presence or absence of 15, 30 or 40 mM oxamate. Data are plotted as the mean ± standard deviation.