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Figure 2 | Breast Cancer Research

Figure 2

From: Wnt signalling in human breast cancer: expression of the putative Wnt inhibitor Dickkopf-3 (DKK3) is frequently suppressed by promoter hypermethylation in mammary tumours

Figure 2

DKK3 mRNA expression after in vitro DNA demethylation. (a) In vitro demethylation of the DKK3 promoter. Methylation specific PCR of seven breast cell lines was performed with DNA from cells either untreated (-) or treated with 1 μM 5-aza-2'-deoxycytidine (DAC) and 300 nM trichostatin A (TSA) (+). (b) Re-expression of DKK3 mRNA in breast cell lines after treatment with DAC/TSA. All cell lines initially exhibiting low DKK3 mRNA restored expression of DKK3 compared with the untreated control cells. Induction level (fold change) of DKK3 mRNA expression was determined by realtime PCR.

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Breast Cancer Research

ISSN: 1465-542X

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