Figure 1From: Phosphorylated EGFR and PI3K/Akt signaling kinases are expressed in circulating tumor cells of breast cancer patientsEGFR and HER2 expression in CTCs from breast cancer patients. (a) Representative images of confocal laser scanning microscopic sections of PBMC cytospins stained with pancytokeratins A45 B/B3 (green) and EGFR anti-rabbit (red) antibodies, showing consecutive scanning sections from the upper cytoplasmic region toward the basal attachment site in step sizes of 0.8 μm (magnification: 600×). (b) Representative images of a cytospin, double stained with monoclonal pancytokeratin (A45-B/B3; green) and polyclonal EGFR anti-rabbit (red) antibodies (magnification: 600×). Also shown is quantification of EGFR and CK co-expression in adjuvant and metastatic CTCs. (c) Representative image of cytospin, double stained with polyclonal pancytokeratin and monoclonal HER2 (green) antibodies (magnification: 600×). Quantification of HER2 and CK co-expression in adjuvant and metastatic CTCs. CK, cytokeratin; CTC, circulating tumor cell; EGFR, epidermal growth factor receptor; HER, human epidermal growth factor receptor; PBMC, peripheral blood mononuclear cell.Back to article page