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Table 1 Oligonucleotide primers employed at melting temperatures (TM in °C) used to determine the expression of RLN2, human RXFP1 relaxin receptor (RXFP1), S100A4 and 18S transcripts in human breast cancer cell lines and xenograft tumours.

From: Relaxin reduces xenograft tumour growth of human MDA-MB-231 breast cancer cells

Primer size primer sequence (5'-3') TM cycles
S100A4 289 bp sense – gaa ggc cct gga tgt gat ggt g 65°C 35
   antisense – cat ttc ttc ctg ggc tgc tta tc   
RLN2 485 bp sense – tct gtt tac tac tga acc aat tt 55°C 35
   antisense – cat ggc aac att tat tag cca a   
RXFP1 243 bp sense – ccc aat tct cta tac tct gac cac aag 65°C 35
   antisense – tca tga ata gga att gag tct cgt tga tt   
18S 344 bp sense – gtt ggt gga gcg att tgt ctg g 62°C 10
   antisense – agg gca ggg act taa tca acg c