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Figure 5 | Breast Cancer Research

Figure 5

From: Relaxin reduces xenograft tumour growth of human MDA-MB-231 breast cancer cells

Figure 5

Long-term exposure to relaxin does not stimulate proliferation in MDA-MB-231. Proliferation of MDA/EGFP and MDA/RLN2 transfectants was determined with (a) bromodeoxyuridine (BrdU) and (b) NADPH2-dependent MTT proliferation assays. Experiments were performed in triplicates and shown in the graph as mean ± SEM. The t-test features of the SPSS 10.0 software were employed to calculate for statistical significance of MDA/RLN2 compared with MDA/EGFP. p < 0.05 was marked in the graph as reduction (*) or as increase (#). **p < 0.005. (c) A luminometric ATP assay was performed to assess the metabolic activity and no changes were determined in MDA/RLN2 compared with MDA/EGFP clones.

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