Top functional categories and canonical pathways identified by IPA. (a) Top eight functional categories from our data set based on significance. The red line (T) indicates the threshold of – logP greater than 2.0. The total numbers of genes (for example 375) and the relative numbers of significant hypomethylated and hypermethylated genes are shown for each category. (b) Top five pathways from the Ingenuity Pathways Analysis library of canonical pathways that were most significant to our data set. For each canonical pathway, hypomethylated (yellow) and hypermethylated (blue) genes are shown (for example 15 and 37), as is the total number of genes in that pathway (for example 193). The top axis represents the percentage of genes per pathway, and the bottom axis the significance (red data points).