Real-time reverse transcription-polymerase chain reaction validation of PN miRNA loss in breast and lymphoblastoid (LB) cell lines. (a) Expression of the listed miRNA genes in 11 additional breast cancer and 2 immortalised normal cell lines alongside 6 of the initial panel of tested cell lines (red). The luminal/basal nature of each cell line is indicated on the right. Values were normalised to the GEOmean of miRNAs 93 and 191 . Data were scaled to the highest-expressing sample for each miRNA. PN miRNAs appear in boldface. (b) The expression of the listed miRNA genes in B cells from 3 healthy blood donors and 3 LB cell lines. Values were normalised to the levels of 5S rRNA and scaled as above. The levels of miRNAs 127, 221, 199a-s, 199a-as, 143, and 145 were independently tested twice. PN miRNAs appear in boldface. miR-214 was below the detection threshold in all samples. PN miRNA, microRNA specifically expressed in PMC42 and Normal breast tissue.