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Table 1 Surface phenotype and mammosphere culture of cells from pleural effusions

From: Mammosphere culture of metastatic breast cancer cells enriches for tumorigenic breast cancer cells

Sample Average size of mammospheres (μm) Population of CD44+/CD24low/- cells (percentage)
PE33 No mammospheresa 14.2
PE61 No mammospheres  
PE70 No mammospheres 37
PE75 No mammospheres 62
PE77 No mammospheres 18.8
PE78 No mammospheres 10.4
PE89 No mammospheres  
PE19 20 67.9
PE20 20 0.3
PE22 20  
PE23 20 65
PE62b 20 13.2
PE63 20  
PE88b 20 37
PE6b 30 1.5
PE79 40 4.8
PE11 50 26
PE43b 50 9.9
06PE5c 50  
06PE7c 50  
PE73 60 29.4
06PE4c 60 4.9
06PE8b,c 70 70.6
PE14b 100 Uncultured cells uniformly CD44- and CD24-
PE21 100 3.3
06PE6b,c 100 15.3
PE66b 130 6.85
  1. Cells from pleural effusion (PE) samples taken from breast cancer patients were tested for their ability to form mammospheres. Uncultured cells were analysed for surface expression of CD24 and CD44 (Figure 3). a7/27 (26%) did not make mammospheres or could not be passaged beyond passage 2. bTested for tumorigenicity in severe combined immunodeficiency disease mice (Table 2). cFive samples obtained in 2006 were placed into culture without freezing. The other 22 samples obtained from 1977 to 1984 had been stored in liquid nitrogen.