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Table 3 Primer sequences for MSP

From: Estrogen receptor and HER2/neu status affect epigenetic differences of tumor-related genes in primary breast tumors

Gene Methylated/unmethylated Direction Sequence
RASSF1A Methylated Forward 5'-GTGTTAACGCGTTGCGTATC-3'
   Reverse 5'-AACCCCGCGAACTAAAAACGA-3'
  Unmethylated Forward 5'-TTTGGTTGGAGTGTGTTAATGTG-3'
   Reverse 5'-CAAACCCCACAAACTAAAAACAA-3'
CCND2 Methylated Forward 5'-TACGTGTTAGGGTCGATCG-3'
   Reverse 5'-CGAAAACATAAAACCTCCACG-3'
  Unmethylated Forward 5'-GTTATGTTATGTTTGTTGTATG-3'
   Reverse 5'-TAAAATCCACCAACACAATCA-3'
GSTP1 Methylated Forward 5'-TTCGGGGTGTAGCGGTCGTC-3'
   Reverse 5'-GCCCCAATACTAAATCACGACG-3'
  Unmethylated Forward 5'-GATGTTTGGGGTGTAGTGGTTGTT-3'
   Reverse 5'-CCACCCCAATACTAAATCACAACA-3'
APC Methylated Forward 5'-TATTGCGGAGTGCGGGTC-3'
   Reverse 5'-TCGACGAACTCCCGACGA-3'
  Unmethylated Forward 5'-GTGTTTTATTGTGGAGTGTGGGTT-3'
   Reverse 5'-CCAATCACAAACTCCCAACAA-3'
TWIST Methylated Forward 5'-TTTCGGATGGGGTTGTTATCG-3'
   Reverse 5'-GACGAACGCGAAACGATTTC-3'
  Unmethylated Forward 5'-TTGGATGGGGTTGTTATTGT-3'
   Reverse 5'-ACCTTCCTCCAACAAACACA-3'
NES1 Methylated Forward 5'-TTCGAAGTTTATGGCGTTTC-3'
   Reverse 5'-TTATTTCCGCAATACGCGAC-3'
  Unmethylated Forward 5'-TTGTAGAGGTGGTGTTGTTT-3'
   Reverse 5'-CACACAATAAAACAAAAAACCA-3'
RARβ2 Methylated Forward 5'-GAACGCGAGCGATTCGAGT-3'
   Reverse 5'-GACCAATCCAACCGAAACG-3'
  Unmethylated Forward 5'-GGATTGGGATGTTGAGAATGT-3'
   Reverse 5'-CAACCAATCCAACCAAAACAA-3'
CDH1 Methylated Forward 5'-TTAGGTTAGAGGGTTATCGCGT-3'
   Reverse 5'-TAACTAAAAATTCACCTACCGAC-3'
  Unmethylated Forward 5'-TAATTTTAGGTTAGAGGGTTATTGT-3'
   Reverse 5'-CACAACCAATCAACAAC ACA-3'
  1. MSP, methylation-specific PCR.