Table 3 Primer sequences for MSP
Gene | Methylated/unmethylated | Direction | Sequence |
|---|---|---|---|
RASSF1A | Methylated | Forward | 5'-GTGTTAACGCGTTGCGTATC-3' |
| Â | Â | Reverse | 5'-AACCCCGCGAACTAAAAACGA-3' |
| Â | Unmethylated | Forward | 5'-TTTGGTTGGAGTGTGTTAATGTG-3' |
| Â | Â | Reverse | 5'-CAAACCCCACAAACTAAAAACAA-3' |
CCND2 | Methylated | Forward | 5'-TACGTGTTAGGGTCGATCG-3' |
| Â | Â | Reverse | 5'-CGAAAACATAAAACCTCCACG-3' |
| Â | Unmethylated | Forward | 5'-GTTATGTTATGTTTGTTGTATG-3' |
| Â | Â | Reverse | 5'-TAAAATCCACCAACACAATCA-3' |
GSTP1 | Methylated | Forward | 5'-TTCGGGGTGTAGCGGTCGTC-3' |
| Â | Â | Reverse | 5'-GCCCCAATACTAAATCACGACG-3' |
| Â | Unmethylated | Forward | 5'-GATGTTTGGGGTGTAGTGGTTGTT-3' |
| Â | Â | Reverse | 5'-CCACCCCAATACTAAATCACAACA-3' |
APC | Methylated | Forward | 5'-TATTGCGGAGTGCGGGTC-3' |
| Â | Â | Reverse | 5'-TCGACGAACTCCCGACGA-3' |
| Â | Unmethylated | Forward | 5'-GTGTTTTATTGTGGAGTGTGGGTT-3' |
| Â | Â | Reverse | 5'-CCAATCACAAACTCCCAACAA-3' |
TWIST | Methylated | Forward | 5'-TTTCGGATGGGGTTGTTATCG-3' |
| Â | Â | Reverse | 5'-GACGAACGCGAAACGATTTC-3' |
| Â | Unmethylated | Forward | 5'-TTGGATGGGGTTGTTATTGT-3' |
| Â | Â | Reverse | 5'-ACCTTCCTCCAACAAACACA-3' |
NES1 | Methylated | Forward | 5'-TTCGAAGTTTATGGCGTTTC-3' |
| Â | Â | Reverse | 5'-TTATTTCCGCAATACGCGAC-3' |
| Â | Unmethylated | Forward | 5'-TTGTAGAGGTGGTGTTGTTT-3' |
| Â | Â | Reverse | 5'-CACACAATAAAACAAAAAACCA-3' |
RARβ2 | Methylated | Forward | 5'-GAACGCGAGCGATTCGAGT-3' |
| Â | Â | Reverse | 5'-GACCAATCCAACCGAAACG-3' |
| Â | Unmethylated | Forward | 5'-GGATTGGGATGTTGAGAATGT-3' |
| Â | Â | Reverse | 5'-CAACCAATCCAACCAAAACAA-3' |
CDH1 | Methylated | Forward | 5'-TTAGGTTAGAGGGTTATCGCGT-3' |
| Â | Â | Reverse | 5'-TAACTAAAAATTCACCTACCGAC-3' |
| Â | Unmethylated | Forward | 5'-TAATTTTAGGTTAGAGGGTTATTGT-3' |
| Â | Â | Reverse | 5'-CACAACCAATCAACAAC ACA-3' |