Figure 4
JS-K, but not JS-43-126, decreases the invasiveness of breast cancer cells across Matrigel. (a) MDA-MB-231, F10, and MCF-7/COX-2 cells were added into Matrigel-coated transwell inserts and incubated with JS-K or JS-43-126 for 72 hours. Cells that invaded through the pores onto the lower side of the filter were fixed, stained, and photographed. (b) The number of invaded cells for each filter was counted in five fields. The invasiveness of the cells is expressed as the mean number of cells that invaded to the lower side of the filter. Columns indicate the mean of triplicate wells ± standard deviation. *Significant decrease in the number of invaded cells relative to untreated cells, P < 0.05. (c) MDA-MB-231, F10, and MCF-7/COX-2 cells were plated on Matrigel-coated wells and incubated with JS-K. After 72 hours, cell proliferation was determined by Celltiter 96® AQueous nonradioactive cell proliferation assay. Columns indicate the mean of pentaplicate wells ± standard deviation. (d) MDA-MB-231 and F10 cells were added into type I collagen-coated transwell inserts and incubated with JS-K for 72 hours. Cells that invaded through the pores onto the lower side of the filter were fixed, stained, and photographed.