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Figure 4 | Breast Cancer Research

Figure 4

From: TNK2 preserves epidermal growth factor receptor expression on the cell surface and enhances migration and invasion of human breast cancer cells

Figure 4

Downregulation of TNK2 by siRNA inhibits human breast cancer cell migration. (a) MDA-MB-231 cells treated with TNK2 targeting SiRNA (S1, S3) had less ability to migrate relative to the nontargeting SiRNA control (N) as measured by a scratch-would healing assay. There was a significant difference between nontargeting SiRNA control (N) and TNK2-targeting SiRNA (S1) at 4, 8 and 24 hours (P = 0.0195, P = 0.0004 and P = 0.0002, respectively). There was a significant difference between nontargeting SiRNA control siRNA (N) and TNK2-targeting SiRNA (S3) at 8 hours (P = 0.032). Lower panels: representative pictures of the scratch-would assays 48 hours following wounding. (b) Proliferation as measured by an Alamar Blue assay was not significantly affected in cells treated with the TNK2-targeting SiRNA (S1, S3) relative to the nontargeting control siRNA (N) over a period of 72 hours. (c) Caspase-dependent apoptosis as measured by a caspase-3 cleavage assay showed no significant difference between cells treated with the TNK2-targeting SiRNA (S1, S3) relative to the nontargeting control siRNA (N) over a period of 72 hours. Taxol is used as a positive control (TAX) and showed significant differences in all cases. (d) There was no significant difference seen for caspase-independent apoptosis as measured by Hoechst staining between cells treated with the TNK2-targeting SiRNA (S1, S3) relative to the nontargeting control siRNA (N) over a period of 72 hours. Taxol is used as a positive control (TAX) and showed significant differences in all cases. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

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