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Table 1 BRCA-1 Oligonucleotides used for binding and shift assays

From: Involvement of a specificity proteins-binding element in regulation of basal and estrogen-induced transcription activity of the BRCA1gene

Promoter element

Oligonucleotides

AP-1/CRE

Sense: 5'-AACCTGAGAGGCGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACGCCTCTCAGGTT-3';

Sp/AP-1/CRE

Sense: 5'-GGGTAGGGGCGGAACCTGAGAGGCGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACGCCTCTCAGGTTCCGCCCCTACCC-3';

Sp-Mut/AP-1/CRE

Sense: 5'-GGGTAGGctaaGAACCTGAGAGGCGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACGCCTCTCAGGTTCttagCCTACCC-3'

Sp/AP-1-Mut/CRE

Sense: 5'-GGGTAGGGGCGGAACtatAGAGGCGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACGCCTCTataGTTCCGCCCCTACCC-3';

 

Sense: 5'-GGGTAGGGGCGGAACCactaAGGCGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACGCCTtagtGGTTCCGCCCCTACCC-3';

Intervening AP-1/CRE sequences:

Sense: 5'-GGGTAGGGGCGGAACCTGAtctGCGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACGCagaTCAGGTTCCGCCCCTACCC-3';

 

Sense: 5'-GGGTAGGGGCGGAACCTGAGAttaGTAAGGCGT-3',

 

Antisense: 5'-ACGCCTTACtaaTCTCAGGTTCCGCCCCTACCC-3';

Sp/Ap-1/CRE-Mut

Sense: 5'-GGGTAGGGGCGGAACCTGAGAGGCtgcAGGCGT-3',

 

Antisense: 5'-ACGCCTgcaGCCTCTCAGGTTCCGCCCCTACCC-3';

 

Sense: 5'-GGGTAGGGGCGGAACCTGAGAGGCGTcttGCGT-3',

 

Antisense: 5'-ACGCaagACGCCTCTCAGGTTCCGCCCCTACCC-3';

  1. AP, activator protein; CRE, cAMP response element; Sp, specificity protein.