Figure 2

Contrast enhancement of a highly vascularized melanoma (MDA-MB435). (a) Fluorescence reflectance images were repetitively acquired up to 24 hours after injection of SIDAG (2 μmol/kg body weight). Note the strong enhancement of the tumour nodule, becoming increasingly conspicuous over the first 3 hours. Shown is a white light image, and fluorescence reflectance imaging images at 30, 60, 120 and 180 minutes after injection. (b) Quantitative data analysis revealed a rapid increase in the contrast to noise ratio (CNR) over the first 3 hours followed by a gradual wash out of the fluorochrome from the tumour tissue.