|
Tissue type
|
Total assessed
|
Stained positive
|
Percent positive
|
IHC Scores
|
|---|
|
Normal
|
5
|
4
|
80
|
1.6
|
|
Benign
|
25
|
12
|
48
|
0.54
|
|
IDC stage I and II
|
12
|
6
|
50
|
0.45
|
|
IDC stage III
|
6
|
1
|
17
|
0.108
|
|
IDC with lymph node metastasis
|
6
|
0
|
0
|
0
|
- A breast carcinoma progression array (CC08-00-001; developed by Cybrdi Inc.) was stained by 1:100 dilution (10 ug/ml) of DNAJB6 monoclonal antibody. Mouse IgG1 was used for the isotype background control. Photomicrographs were taken in the area of most intense and diffuse staining for mammalian relative of DnaJ (MRJ). The intensity of staining of tumor cells was assessed as 0 (no staining) to 3 (strongest possible intensity of staining). The immunoscore was derived as the product of the percentage of cells at each intensity and the corresponding intensity [18]. The products were added to obtain an average immunoscore for the group. IDC, infiltrating ductal carcinoma; IHC, immunohistochemistry.
