Figure 3

Digital methylation-sensitive high resolution melting (MS-HRM) and sequencing for KCF3 peripheral blood DNA. The blue curve indicates the unmethylated control. The black curve indicates the methylated control. The green curves indicate unmethylated amplicons. The red curves indicate methylated amplicons. Sequencing for the unmethylated and methylated controls are shown above the digital MS-HRM results. Sequences of the indicated red amplicons (lettered) are shown to right of the figure where the letter to the right of the chromatograph corresponds to the curve shown.