Gene | A1.8: SC+/SC- | RP1: CD133+/CD133- |
|---|
T
| >20 | 4.6 |
Sox1
| 19.2 | 2.2 |
Aldh1a1
| 3.5 | 3.2 |
Fgfr1
| 3.2 | 2.1 |
Tert
| 1.2 | 4.4 |
Notch1
| 2.5 | 4.1 |
CD44
| 1.9 | 3.4 |
Ascl2
| 7.2 | Â |
Pdx1
| 7.1 | Â |
Acan
| 6.6 | Â |
Dhh
| 4.3 | Â |
ALDH2
| Â | 3 |
MME
| Â | 4.5 |
KRT15
| Â | 17 |
JAG1
| Â | 2.2 |
FOXa1
| Â | 19 |
DLL1
| Â | 6 |
DLL3
| Â | 3 |
COL9a1
| Â | 3 |
CDH1
| Â | 11 |
CD8B
| Â | 6 |
CCNA2
| Â | 2.5 |
- Expression of genes from a panel of 84 stem cell-associated genes was determined by SuperArray real-time PCR using RNA from A1.8 and RP.1 cells sorted for CD44, CD24, or CD133 markers (SC+ and SC-). For a complete list of genes tested, see [48]. The results are expressed as fold increase in cells sorted positive over negative markers from replicate experiments. Data are normalized to five housekeeping genes, as per the manufacturers' instructions. Genes common for both cell lines are presented at the top.
