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Table 2 Sequences of small interfering RNAs and primers for reverse transcription-polymerase chain reaction

From: The combined immunodetection of AP-2α and YY1 transcription factors is associated with ERBB2 gene overexpression in primary breast tumors

 

Sequence 5'-3'

Location

siRNAs

  

   siAP-2α ss

CCGAAUUUCCUGCCAAAGCdTdT

 

   siAP-2α as

GCUUUGGCAGGAAAUUCGGdTdT

 

   siAP-2γ ss

UUAAAUAUUCUGCCACUGGdTdT

 

   siAP-2γ as

CCAGUGGCAGAAUAUUUAAdTdT

 

   siYY1 ss

GAACUCACCUCCUGAUUAUdTdT

 

   siYY1 as

AUAAUCAGGAGGUGAGUUCdTdT

 

RT-PCR primers

  

   AP-2α forward

AGCTGAATTTCTCAACCGACAAC

1,013 (exon 5)

   AP-2α reverse

TAGCCAGGAGCATGTTTTTTCTT

1,083 (exon 6)

   AP-2γ forward

CAGAAGAGCCAAATCGAAAAATG

1,041 (exons 5–6)

   AP-2γ reverse

ATTCAACCCAATCTTGTCCAACTT

1,107 (exon 6)

   ERBB2 forward

CTGAACTGGTGTATGCAGATTGC

2,617 (exon 20)

   ERBB2 reverse

TTCCGAGCGGCCAAGTC

2,699 (exon 21)

  1. as, antisense strand; RT-PCR, reverse transcription-polymerase chain reaction; siRNA, small interfering RNA; ss, sense strand.
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Breast Cancer Research

ISSN: 1465-542X

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