Figure 4

Analysis of derlin-1 expression in human breast cancer cell lines. (a) Western blot analysis of derlin-1 protein in T47D, MDA-MB-453, MCF-7, SKBR-3, MDA-MB-435, and 1590 cells. (b) Western blot analysis of derlin-1 protein in T47D, MDA-MB-453, and MDA-MB-435 cells that were exposed to 2 μg/mL tunicamycin (TM) and 300 nM thapsigargin (TG) for 24 hours. (c) Reverse transcription-polymerase chain reaction analysis of derlin-1 expression in T47D cells that were exposed to TM, TG, and serum starvation. (d) Western blot analysis of derlin-1 protein in T47D cells that were exposed to serum starvation. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.