Figure 5

NHERF1 increases cell sensitivity to STI-571. (a) Zr75.1 cells with NHERF1 knockdown (910) or Babe controls were cultured in 96-well plates and treated with STI-571 (10 and 30 μmol/l) for 2 days. (b) MCF10A cells over-expressing Na⁺/H⁺ exchanger regulatory factor 1 (NHERF1) or vector control were subjected to STI-571 treatment (3 and 10 μmol/l) for 2 days. MTT reagents were added to assess the relative number of surviving cells. The numbers of untreated Babe control were arbitrarily set at 100%. Data from five independent experiments are presented. **P < 0.01, ***P < 0.001 versus Babe control of the corresponding treatment.