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Figure 8 | Breast Cancer Research

Figure 8

From: Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells

Figure 8

Effect of calcium on ibandronate-induced inhibition of Rap1A prenylation in MDA-MB-231 and MCF-7 cells. Cells were incubated for 24 hours with increasing concentrations of ibandronate (1 to 1,000 μmol/l) in culture medium containing 0.6 or 2.0 mmol/l calcium, lysed and subjected to Western blot analysis. Equal amounts of proteins (25 μg) were subjected to 12% SDS-PAGE and electrotransferred onto nitrocellulose membranes. Immunodetection was performed with a goat polyclonal anti-human antibody raised against the unprenylated form of Rap1A. Total Rap1 protein (input) was evaluated in parallel using a rabbit polyclonal anti-human Rap1 antibody. Representative data from two separate experiments.

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