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Figure 5 | Breast Cancer Research

Figure 5

From: Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells

Figure 5

Effect of EGTA on growth inhibition induced by ibandronate in presence of high calcium concentration. MDA-MB-231 and MCF-7 cells were treated for 72 hours with 30 μmol/l ibandronate (Iban) alone or with 500 μmol/l ethylene glycol tetra-acetic acid (EGTA) in culture medium containing 1.6 mmol/l calcium. Cell culture densities were determined by crystal violet staining assay. Data are presented as percentages (mean ± standard deviation) of control values, which refer to culture with 1.6 mmol/l calcium and without drugs. Mean of results pooled from two experiments (n = 12). *P < 0.01 between indicated conditions, analysis of variance; #P < 0.01 versus control, analysis of variance; Tukey post hoc test.

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