Figure 3
From: Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells
Effect of calcium on cell apoptosis induced by bisphosphonates in MDA-MB-231 and MCF-7 cells. Cells were incubated for 72 hours with 30 μmol/l ibandronate (Iban) or zoledronic acid (Zole) in culture medium containing 0.6 or 1.6 mmol/l calcium. Apoptotic cell death was assessed by annexin V-phycoerythrin (PE) and 7-amino-actinomycin (7-AAD) double staining. Data are presented as percentages (mean ± standard deviation) of annexin V-PE-positive (AN+) and 7-AAD-negative cells. Mean of results pooled from two experiments (n = 4). *P < 0.01 versus 0.6 mmol/l calcium, analysis of variance, Tukey post hoc test.