Figure 8From: Silencing of GM3 synthase suppresses lung metastasis of murine breast cancer cellsExpression of NFAT1 was inhibited through the PI3K/Akt pathway in GM3S-knockdown 4T1 cells. (a) Upper panels: expression of NFAT1 was detected by immunoblotting. Lower panels: quantitation of these blots after normalization with the GAPDH blot. (b) Upper panels: expression of NFAT1 was enhanced by the PI3K inhibitor LY294002. Lower panels: quantitation of these blots after normalization with the GAPDH blot. *P < 0.05, **P < 0.01. CMP, cytidine 5'-monophosphate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GM3S, CMP-N-acetylneuraminic acid:lactosylceramide 2,3-sialyltransferase; NFAT, nuclear factor of activated T cell; PI3K, phosphoinositide-3 kinase.Back to article page