Table 1 Details of primary antibodies used
Antibody | Details of antibody | Antigen retrieval | Dilution | Incubation period (hours) | Reference |
|---|---|---|---|---|---|
Factor-inhibiting hypoxia-inducible factor 1 (FIH162c) | Mouse monoclonal IgG2k (Nuffield Department of Clinical Laboratory Sciences, Oxford, UK) | None | Neat supernatant | 1.5 | [23] |
Hypoxia-inducible factor 1α (ESEE122) | Mouse monoclonal IgG1 (Nuffield Department of Clinical Laboratory Sciences) | Pressure cook for 3 min in ethylenediamine tetraacetic acid, pH 8.0 | 1:100 | 4 | [24] |
Carbonic anhydrase 9 (M75) | Mouse monoclonal (from J. Pastorek, Institute of Virology, Slovak Republic) | None | 1:50 | 0.5 | [25] |