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Table 1 Details of primary antibodies used

From: Cytoplasmic location of factor-inhibiting hypoxia-inducible factor is associated with an enhanced hypoxic response and a shorter survival in invasive breast cancer

Antibody Details of antibody Antigen retrieval Dilution Incubation period (hours) Reference
Factor-inhibiting hypoxia-inducible factor 1 (FIH162c) Mouse monoclonal IgG2k (Nuffield Department of Clinical Laboratory Sciences, Oxford, UK) None Neat supernatant 1.5 [23]
Hypoxia-inducible factor 1α (ESEE122) Mouse monoclonal IgG1 (Nuffield Department of Clinical Laboratory Sciences) Pressure cook for 3 min in ethylenediamine tetraacetic acid, pH 8.0 1:100 4 [24]
Carbonic anhydrase 9 (M75) Mouse monoclonal (from J. Pastorek, Institute of Virology, Slovak Republic) None 1:50 0.5 [25]