Table 1 Primers and conditions used for the newborn DNA population prevalence study
Mutation | Primers (5' to 3') | Wild-type allele conditions | Mutated allele conditions |
|---|---|---|---|
Q775X | ACCAGTGGAGCCCTTTGAGTC-common primer GCCTGAACTGTCGAATTG-wild-type allele GCCTGAACTGTCGAATTA-mutated allele | Annealing temperature of 59°C for 35 cycles | Annealing temperature of 59°C for 36 cycles |
I76V | TAGTCGCCCTGGTGAAATTAG-common primer CTCAGAACCTAAAAATAAAA-wild-type allele CTCAGAACCTAAAAATAAAG-mutated allele | Annealing temperature of 51°C for 36 cycles | Annealing temperature of 55°C for 38 cycles |
G115V | TACTTGAGCCAAGGGGGAAAA-common CTGTTCTTTGTATAGGTAATC-wild-type allele CTGTTCTTTGTATAGGTAATA-mutated allele | Annealing temperature of 53°C for 35 cycles | Annealing temperature of 53°C for 39 cycles |