Figure 1From: Identification of BRCA1 missense substitutions that confer partial functional activity: potential moderate risk variants?BRCA1 R1699Q causes destabilisation of the BRCT domain. In vitro-transcribed and -translated BRCA1 cDNA fragments containing wild-type or unclassified variant sequence, incorporating sulfur-35-labelled methionine, were treated with increasing concentrations of trypsin (μg/mL) and resolved on SDS-PAGE.Back to article page