Figure 1
From: Mouse mammary tumors display Stat3 activation dependent on leukemia inhibitory factor signaling
Expression of LIF and LIF receptor mRNA in mammary tumor cells. (a) Real-time PCR showing relative expression levels of leukemia inhibitory factor (LIF) in hormone-dependent (HDT) and hormone-independent (HIT) mouse mammary tumors compared with lactating (Lac) and 48-hour involuting (Inv) normal mammary glands. All samples were analyzed and normalized to actin expression in parallel in the same real-time PCR assay. (b) Ethidium bromide stained gel showing RT-PCR analysis of two different LIF splicing variants: LIF-M and LIF-D. (c) Ethidium bromide stained gel showing LIF and LIF-R expression by RT-PCR in HDT, HIT and in culture growing mammary cell lines: NMuMG, HC11, SCp2 (epithelial non-tumorigenic), MCF-7, LM3, LMM3 (tumorigenic mammary cell lines) and mammary tumor primary cultures (TPC).