Figure 5
Role of exon 1 in the activity of the 5' flanking region of HGFIN. (a) Cartoon of the upstream region of HGFIN. TS, transcription start site. (b) Non-tumorigenic (MCF12A) and tumorigenic (T47D, HCC70, MDA-MB-231) breast cells were co-transfected with pGL3-HGFIN-RM/2.0 or -RM/2.0E and pGal. Luciferase activities were normalized with β-galactosidase activities and the data are presented as the mean ± SD, n = 6. *p < 0.05 vs HGFIN-RM/2.0; **p > 0.05 vs HGFIN-RM/2.0E, MDA-MB-231. (c) T47D were co-transfected with HGFIN-RM/2.0 or HGFIN-RM/2.0E, and wild-type or mutant p53 expression vectors. Controls were transfected with vector alone. Luciferase activities were determined 16 h after transfection. The data are presented as mean ± SD, n = 6.