Figure 1

HGFIN expression in breast cells and tissues. (a) RT-PCR for HGFIN and GAPDH in non-tumorigenic (NT) (MCF10 and MCF12A) and tumorigenic (T) (DU4475) breast cells. P1, P10 and P38 are the non-identifier codes for patients. (b) Total RNA was analyzed by northern analyses for HGFIN from MCF12A non-tumorigenic cells and tumorigenic cell lines (HCC70 and T47D). Membranes were stripped and re-probed for 18S rRNA. (c) and (d) Representative in situ hybridizations for HGFIN from benign breast tissue, n = 25 (c) and malignant cells, n = 50 (d). Arrow shows dense staining for alkaline phosphatase (blue) in the benign section. The images were acquired with 100×/0.3 NA objectives.