Figure 1
The cytotoxic and growth inhibitory effects of CDIM9 in MD-MB231 and BT549 cells. (a) MDA-MB231 cells, SKMCs, BT549 cells, and HSMMs were treated with serial diluted CDIM9 at between 1 × 10-3 and 1.9 × 10-6 mol/l for 48 hours. The EC50 values were determined using GraphPad Prism software. (b) Cell growth inhibition assay. MD-MB231 and BT549 cells were treated with 1 to 10 μmol/l CDIM9 for 6 days, and cell numbers were determined as described in Materials and methods. Results are expressed as means ± standard error for three separate determinations at each time point. CDIM9, 1,1-bis (3'-indolyl)-1-(p-biphenyl) methane; EC50, concentration producing 50% of the maximum possible response; HSMM, human muscle myoblast; SKMC, human skeletal muscle cell.