Effects of steroidal and nonsteroidal aromatase inhibitors on markers of bone turnover in healthy postmenopausal women

Table 1 Laboratory methods for assay of bone-turnover markers, lipids, and estrogens

Parameter	Method	Manufacturer
Bone resorption
Adjusted U-CTx	Enzyme immunoassay test using the CrossLaps^® ELISA kit	Nordic Bioscience Diagnostics A/S, Herlev, Denmark
S-CTx	Electrochemoluminescence immunoassay technique using Elecsys 2010 automate analyzer	Roche Diagnostics, Basel Switzerland
Adjusted U-NTx	Competitive immunoassay technique using the NTx reagent pack kit	Ortho-Clinical Diagnostics, Amersham, UK
Bone formation
BAP	Ostase Assay using Access Immunoassay System	Beckman Coulter, Villepinte, France
PINP	Electrochemoluminescence immunoassay technique using Elecsys 2010 automate analyzer	Roche Diagnostics
Lipids (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides)	Validated HPLC–RIA technique using Bayer Advia Reagent Packs. Assayed on an ADVIA^® 1650 chemistry system analyzer	Bayer Diagnostics, Bayer Inc., Tarrytown, NY, USA
Estrogen (E₁, E₂, and E₁S)	Validated HPLC–RIA technique	Aster-Cephac, Paris, France

BAP, bone alkaline phosphatase; E₁, estrone; E₁S, estrone sulfate; E₂, estradiol; ELISA, enzyme-linked immunosorbent assay; HDL, high-density lipoprotein; HPLC–RIA, high-performance liquid chromatography radioimmunoassay; LDL, low-density lipoprotein; PINP, procollagen type I N-terminal propeptide; S-CTx, serum C-terminal telopeptide of type I collagen; U-CTx, urine-adjusted urinary C-terminal telopeptide of type I collagen; U-NTx, urine-adjusted urinary N-terminal telopeptide of type I collagen.

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