Skip to main content

Table 1 Laboratory methods for assay of bone-turnover markers, lipids, and estrogens

From: Effects of steroidal and nonsteroidal aromatase inhibitors on markers of bone turnover in healthy postmenopausal women

Parameter Method Manufacturer
Bone resorption
   Adjusted U-CTx Enzyme immunoassay test using the CrossLaps® ELISA kit Nordic Bioscience Diagnostics A/S, Herlev, Denmark
   S-CTx Electrochemoluminescence immunoassay technique using Elecsys 2010 automate analyzer Roche Diagnostics, Basel Switzerland
   Adjusted U-NTx Competitive immunoassay technique using the NTx reagent pack kit Ortho-Clinical Diagnostics, Amersham, UK
Bone formation
   BAP Ostase Assay using Access Immunoassay System Beckman Coulter, Villepinte, France
   PINP Electrochemoluminescence immunoassay technique using Elecsys 2010 automate analyzer Roche Diagnostics
Lipids (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides) Validated HPLC–RIA technique using Bayer Advia Reagent Packs. Assayed on an ADVIA® 1650 chemistry system analyzer Bayer Diagnostics, Bayer Inc., Tarrytown, NY, USA
Estrogen (E1, E2, and E1S) Validated HPLC–RIA technique Aster-Cephac, Paris, France
  1. BAP, bone alkaline phosphatase; E1, estrone; E1S, estrone sulfate; E2, estradiol; ELISA, enzyme-linked immunosorbent assay; HDL, high-density lipoprotein; HPLC–RIA, high-performance liquid chromatography radioimmunoassay; LDL, low-density lipoprotein; PINP, procollagen type I N-terminal propeptide; S-CTx, serum C-terminal telopeptide of type I collagen; U-CTx, urine-adjusted urinary C-terminal telopeptide of type I collagen; U-NTx, urine-adjusted urinary N-terminal telopeptide of type I collagen.