Skip to main content

Table 2 Effect of Wilms' tumor 1 suppressor gene (WT1) plasmid with ZF - KTS on the growth of breast cancer cells

From: The zinc finger domain of Wilms' tumor 1 suppressor gene (WT1) behaves as a dominant negative, leading to abrogation of WT1 oncogenic potential in breast cancer cells

Cells

pcDNA3

pcDNA3/ZF - KTS

pcDNA3/WT1

pSuper

pSuper/WT1

MCF-7

     

   Mean ± standard deviation (CFU)

142 ± 3.3

51 ± 4*

164 ± 9*

51 ± 4.8

40 ± 3.7*

   Relative change (%)

0

-64.1

+15.5

0

-21.8

MDA468

     

   Mean ± standard deviation (CFU)

291 ± 8.7

214 ± 15.3*

406 ± 17*

177 ± 6.1

42 ± 1.7*

   Relative change (%)

0

-26.5

+39.5

0

-76.2

MDA231

     

   Mean ± standard deviation (CFU)

131 ± 6.2

130 ± 3.8

142 ± 5

36 ± 1.1

32 ± 3.0

   Relative change (%)

0

-0.2

+8.8

0

-11.2

  1. Relative change represents the percentage difference in number of colony-forming units (CFU) produced in the zinc finger domain lacking the lysine–threonine–serine insert (ZF - KTS), in full-length WT1 or in WT1 shRNA transfected cells in comparison with empty vector, respectively. *P < 0.05.