Figure 5

Abrogation of WT1-mediated c-myc transactivation by the zinc finger domain lacking KTS in MCF-7 cells. The MCF-7 cells were transiently co-transfected with the reporter constructs (either pGL2 vector, XNM/Luc or Xmut/Luc), with expression plasmids of Wilms' tumor 1 suppressor gene (WT1) isoforms (either pcDNA₃ vector, A, B, C or D) and with WT1–zinc finger domain (ZF) (either pcDNA₃ control (CON) or ZF lacking the lysine–threonine–serine insert (ZF-KTS)) as well as β-galactosidase expression plasmid, respectively. Relative luciferase activity (vertical axis) is indicated. Histograms show average of three independent experiments.