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Figure 7 | Breast Cancer Research

Figure 7

From: An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells

Figure 7

ERα/BMI1/TERT/MYC-transduced HMECs are genetically stable. (a) Representative karyotype of two different human mammary epithelial cell (HMEC) strains infected with oestrogen receptor alpha (ERα), BMI1, TERT and MYC viruses after continuous in vitro passage for 60 doublings. (b) Copy number and loss of heterozygosity (LOH) plots. Each sample has three tracks: smoothed intensity, copy number prediction by Hidden Markov Model and LOH prediction. The horizontal axis shows each chromosome in turn, starting with the short arm of chromosome 1. In the intensity track each chromosome is given a different colour. The first set of tracks is from MCF7 cells, which were used as a positive control to demonstrate that the technique can detect amplification, deletion and LOH. The remaining three sets of tracks are, respectively, cells from reduction mammoplasty tissue of patient AJ at passage 0 (AJ P0), ERα/BMI1/TERT/MYC-transduced HMECs from the same patient just before they were injected into mice at passage 6 (AJ P6), and the same cells after recovery from established tumours (AJ Tumour).

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