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Figure 2 | Breast Cancer Research

Figure 2

From: An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells

Figure 2

Gene expression profiles of HMECs transduced with GUS, ERα and BMI1. Cells transduced with GUS alone, ERα alone, BMI1 alone or ERα and BMI1 together were grown for 24 hours in the presence of oestrogen (E) or the oestrogen antagonist fulvestrant (F) to activate or block oestrogen signalling. Known BMI1 target genes (p16 CDKN2A (a), CCND2 (c) and NEFL (d)) are repressed by BMI1. Endogenous BMI1 is also repressed in BMI1-transduced cells (b) (the probe on the array detects only the endogenous transcript; the Western blots in Figure 1 show that overall BMI1 expression is increased in the BMI1-transduced cells despite the decrease in endogenous BMI1 expression). Many known oestrogen inducible genes are strongly induced by oestrogen receptor alpha (ERα) in the presence of oestrogen (e) GREB1 (gene regulated in breast cancer 1), (f) the prolactin receptor (PRLR) and (g) the progesterone receptor (PGR)), but some barely change ((h) TFF1). GUS, glucuronidase; HMEC, human mammary epithelial cell.

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