Figure 6
The effect of concentrated NMF-conditioned media on two-dimensional scratch wound closure. PMC42-LA cells were cultured on glass in 1×, 2×, 4×, or 10× NMF-conditioned medium. Using scratch tests, PMC42-LA cells were analyzed for the extent of wound closure 24 hours after scratch. Scratches were measured and averaged, and the extent of wound closure calculated and averaged. (a) Cells in 1× NMF-conditioned media (control) had average wound sizes of 307 ± 24.3 μm 24 hours post-scratch. (b) Cells in 2× NMF-conditioned medium had average wound sizes of 319 ± 23.2 μm 24 hours post-scratch. (c) Cells in 4× NMF-conditioned medium had average wound widths of 232 ± 49.7 μm 24 hours post-scratch, and (d) cells in 10× NMF-conditioned medium had average wound widths of 160 ± 62.4 μm 24 hours post-scratch. Vimentin localization appeared unchanged in all cultures; some change in cell morphology was noted in 10× NMF-conditioned media cultures. CAF, cancer-associated fibroblast; NMF, normal mammary fibroblast.