Skip to main content
Figure 2 | Breast Cancer Research

Figure 2

From: Telomeric DNA induces apoptosis and senescence of human breast carcinoma cells

Figure 2

T-oligo decreases cell yield and induces S phase arrest in MCF-7 cells. Preconfluent cultures of (a) MCF-7 cells and (b) normal mammary epithelial (NME) cells were treated with 40 μM of GTTAGGGTTAG (T-oligo), CTAACCCTAAC (control oligo) or diluent alone and cell yields were determined at different intervals after oligonucleotide addition. Compared to controls, within 4 days T-oligo decreased cell yields 87 ± 9% (MCF-7; p < 0.04, n = 4) and 65.5 ± 0.01% (NME; p < 0.03, n = 2). (c) MCF-7 cells were treated as above and cells were collected for up to 96 hours for FACScan analysis after a single treatment at time 0. Averages and standard deviations were determined from duplicate samples. T-oligo increased the percent of cells in the S phase of the cycle as early as 48 hours after stimulation (p < 0.001, n = 2) and for at least 96 hours after stimulation (p < 0.04, n = 2).

Back to article page