Effect of Na+/H+ exchanger regulatory factor 1 (NHERF1) knockdown on cell cycle distribution. (a) The cell cycle distribution of asynchronous T47D cells (NHERF1 knockdown and Babe control) was determined by fluorescence-activated cell sorting (FACS) analyses. Cells were cultured in media supplemented in 10% foetal bovine serum before being trypsinised, fixed in 75% ethanol, and stained with propidium iodide. (b) Cell cycle distribution of MCF7/NHERF-910 and MCF7/Babe cells was assessed by FACS. Three independent experiments were conducted. Data are presented as means ± standard error. *P < 0.05, **P < 0.01, as compared with Babe cells.