Growth of T47D and MCF7 cells with Na+/H+ exchanger regulatory factor 1 (NHERF1) knockdown. (a) Representative phase-contrast view of monolayer culture of T47D/NHERF-786 cells in comparison with T47D/Babe control, prior to MTT assay. (b) Growth-rate comparison of T47D/NHERF-786 and T47D/Babe cells by MTT assay. Cells cultured in 96-well plates were allowed to grow for 1 to 7 days before MTT reagents were added. The metabolic products of MTT deposited in cells were measured by optical density (O.D.) at 570 nm. (c) MCF7/NHERF-910 and MCF7/Babe cells were measured by MTT assay as described in (b). The growth curve is representative of at least three independent experiments. *P < 0.05 versus Babe cells. #P < 0.05 versus parental cells. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.